Description
Principle of the assay: human Mer ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Mer has been precoated onto 96-well plates. Standards(sf21, R26-A499) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Mer is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mer amount of sample captured in plate.
Background: Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)